Handbook of Phycological Methods
Physiological and Biological Methods

Table of Contents

Contributors
Editors' preface
Introduction
Part I. Isolation of Organelles and Membranes:
1. Chloroplasts from Euglena gracilis C. A. Price
2. Nuclei and chromatin from Euglena gracilis D. E. Buetow
3. Mitochondria from Chlorella Neil G. Grant
4. Microbodies from Chlorogonium and Spirogyra Helmut Stabenau
5. Isolation and characterization of diatom membranes Cornelius W. Sullivan
Part II. Analysis of Chemical Constituents:
6. Chlorophylls and carotenoids Arne Jensen
7. Algal biliproteins Harold W. Siegelman and J. Helen Kycia
8. Nucleic acids Rose Ann Cattolico
9. Protein determination by dye binding Gary Kochert
10. Carbohydrate determination by the phenol-sulfaric acid method Gary Kochert
11. Glycerolipids and fatty acids of algae J. P. Williams
12. Carrageenans and agars J. S. Craigie and C. Leigh
13. β-1,3-Glucans in diatoms and brown seaweeds Sverre Myklestad
14. Alginic Acid Bjørn Larsen
15. Phenolic compounds in brown and red algae Mark A. Ragan and J. S. Craigie
16. Brown seaweeds: analysis of ash, fiber, iodine and mannitol Bjørn Larsen
17. Quantitation of the macromolecular components of microalgae Gary Kochert
18. ATP, ADP, and AMP determinations in water samples and algal cultures David M. Karl and Osmund Holm-Hansen
Part III. Enzymes:
19. Ribulose-bis-phosphate carboxylase in Chlamydomonas S. S. Badour
20. Nitrate reductase in marine phytoplankton Richard W. Eppley
21. Threonine deaminase in marine microalgae N. J. Antia and R. S. Kripps
22. Phosphoenolpyruvate carboxylse of blue-green algae Brian Colman
23. Aldolases of multicellular marine algae Kazutosi Nisizawa
24. The glucosyltranserases of red algae Jerome F. Frederick
25. Anion-activated adenosine triphosphates Paul G. Falkowski
26. Extracellular acid phosphatase of Ocheomonas sanica N. J. Patni and S. Aaronson
Part IV. Physiological and Biochemical Processes:
27. Determination of photosynthetic rates and 14C photoassimilatory products of brown seaweeds Bruno P. Kremer
28. Polargraphic measurements of respiration following light-dark transitions Alan D. Jassby
29. Hill reaction and photophosphorylation with chloroplast perparations from Chlamydomonas reinhardi Stephen Lien
30. Respiratory assimilations of 14C-labelled substrates by a microalga Keith E. Cooksey
31. Respiratory processses in mitochondria Neil G. Grant
32. Determination of protein synthesis during synchronous growth Dennis J. O'Kane and Raymond F. Jones
33. Nucleic acid synthesis Rose Ann Cattolico
34. Nitrogen fixation Timothy H. Mague
35. Uptake of organic substrates Johan A. Hellebust and Yi-Hung Lin
36. Release of organic substances C. Nalewajko
Part V. Nutrients:
37. Nitrate uptake Richard W. Eppley
38. Phosphate uptake F. P. Healey
39. Sulfate uptake Monica Lik-Shing Tsang, Robert C. Hodson and Jerome A. Schiff
40. Uptake of silicic acid Farooq Azam and Sallie W. Chisholm
41. Uptake of micronutrients and toxic metals Anthony G. Davies
Part VI. Ion Content and Transport:
42. Measurement of ionic content and fluxes in microalgae J. Barber
43. Ion transport and ion-stimulated adenosine triphosphatases Cornelius W. Sullivan
Part VII. Inhibitors:
44. Inhibitors used in studies of algal metabolism S. S. Badour
Part VIII. Appendix
Part IX. Indexes.