Haematopoietic and Lymphoid Cell Culture
$62.99 (C)
Part of Handbooks in Practical Animal Cell Biology
- Editors:
- Margaret J. Dallman, Imperial College of Science, Technology and Medicine, London
- Jonathan R. Lamb, University of Edinburgh
- Date Published: August 2000
- availability: Available
- format: Paperback
- isbn: 9780521629690
$
62.99
(C)
Paperback
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This invaluable reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact hematopoietic and lymphoid cells, and their maintenance as primary cultures. The text provides technical information on the signals and mediators required for the differentiation and growth of these cells, and is designed for laboratory investigators with limited practical experience in cell culture. Chapters discuss dendritic cells, T and B lymphocytes, monocytes and macrophages, NK and LAK cells, mast cells and basophils, hematopoietic differentiation of embryonal stem cells, and the culturing of murine thymic explants. Each chapter has been written by experts who have practical experience of the techniques discussed to provide tips for avoiding common pitfalls, and sharing insight into the fundamental questions in cell biology and immunology addressed using each cell culture model.
Read more- Detailed, step-by-step protocols
- Written by experts with first hand experience to avoid common pitfalls
- Includes details of reagents and suppliers
Reviews & endorsements
"The book would be of value to anyone who wishes to grow differentiated hematopoietic and lymphoid cells in culture...This is a useful, practical guide, is reasonably priced, and would be a valuable book to have on a laboratory bookshelf or in a library reference collection of techniques." Doody's Review Service ®
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×Product details
- Date Published: August 2000
- format: Paperback
- isbn: 9780521629690
- length: 204 pages
- dimensions: 229 x 153 x 13 mm
- weight: 0.385kg
- contains: 41 b/w illus.
- availability: Available
Table of Contents
List of contributors
Preface
Introduction
1. Haematopoietic differentiation of embryonic stem cells John Ansell and Nicholas Hole
2. Dendritic cells Jonathan M. Austyn, David Chao, Chen-Lung Lin, Justin A. Roake and Rakesh Suri
3. Murine thymic explant cultures Kim L. Anderson and John J. T. Owen
4. T cells Hergen Spits and Colin G. Brooks
5. B lymphocytes Anne E. Corcoran and Ashok R. Venkitaraman
6. Monocytes and macrophages James A. Mahoney, Richard Haworth and Siamon Gordon
7. NK cells and LAK cells Colin G. Brooks and Hergen Spits
8. Mast cells and basophils Gunnar Nilsson and Dean D. Metcalfe
Index.
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