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Look Inside Fundamentals of Light Microscopy

Fundamentals of Light Microscopy

Part of IUPAB Biophysics Series

  • Date Published: August 1982
  • availability: Available
  • format: Paperback
  • isbn: 9780521289672

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  • Dr Spencer's account gives, in largely non-mathematical terms, a concise but rigorous explanation of the principles underlying the various forms of light microscopy. It starts with the properties of lenses, elementary diffraction theory and theoretical limits to resolution; it goes on to explain the workings of phase contrast, dark-field, fluorescence, polarizing and interference microscopes. There are chapters on aberrations and photomicrography, and detailed instructions for the alignment and general care of microscopes. Questions and answers are given at the end of each chapter, and suggestions made for class experiments. Although written primarily for undergraduates, the treatment is equally suitable for those preparing for professional examinations in microscopy, and for research workers seeking a better understanding of the microscopes they use.

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    Product details

    • Date Published: August 1982
    • format: Paperback
    • isbn: 9780521289672
    • length: 104 pages
    • dimensions: 229 x 152 x 6 mm
    • weight: 0.17kg
    • availability: Available
  • Table of Contents

    Preface
    1. Microscope alignment
    2. Properties of light
    3. The limits of resolution
    4. The Abbe approach
    5. Bright-field, phase and dark-field microscopy
    6. Fluorescence microscopy
    7. Polarizing microscopy
    8. Interference methods
    9. Aberrations and microscope design
    10. Photomicrography
    11. Summary, class experiments and further reading.

  • Author

    Michael Spencer

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