Clinical Gene Analysis and Manipulation
Tools, Techniques and Troubleshooting
Out of Print
Part of Postgraduate Medical Science
- Editors:
- Janusz A. Z. Jankowski, University Hospital, Birmingham
- Julia M. Polak, Royal Postgraduate Medical School, London
- Date Published: September 1996
- availability: Unavailable - out of print April 1999
- format: Hardback
- isbn: 9780521473262
Out of Print
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This practical compendium provides clinical scientists with an essential guide to the basic techniques of molecular medicine. It serves as a laboratory manual and a source of reference. It is suitable for those wishing to perform basic semi-quantitative experiments such as Northern or Southern blots and also those wishing to undertake more specialised genetic manipulations such as gene cloning, expression and creation of DNA libraries. It will give clinical scientists a unique insight into the potential of these techniques. As stated by Sir David Weatherall: 'It should be of great value to both established research workers and young scientists coming into the field for the first time. It deserves every success.'
Read more- Deals with: core knowledge; equipment required; techniques; clinical application
- Bridges the gap from protocol to publication
- Foreword by Sir David Weatherall 'It deserves every success'
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×Product details
- Date Published: September 1996
- format: Hardback
- isbn: 9780521473262
- length: 476 pages
- dimensions: 253 x 179 x 27 mm
- weight: 1.107kg
- contains: 60 b/w illus. 10 tables
- availability: Unavailable - out of print April 1999
Table of Contents
Foreword Sir D. Weatherall
Part I. Introduction, Background and Essential Theory:
1. Introduction and applications J. Jankowski
2. History of molecular genetics R. Chinery
3. Molecules of gene expression (protein, RNA and DNA) R. Chinery
Part II. Molecular Cell Tools:
4. Tissue analysis and morphological characterisation
5. Cellular analysis and cell culture
6. Chromosomal and molecular analysis
7. Gene manipulation F. Bedford and J. Jankowski
Part III. Molecular Cell Techniques:
8. Cellular analysis
Initiation and propagation of cultured cell lines I. Freshney
Characterisation of cultured cell cines S. Kirkland and K. Henderson
Cytoxicity assays in vitro R. Phillips and B. Boullier
The production of monoclonal and polyclonal antibodies using synthetic peptides as immunogens T. RajKumar and B. Gullick
9. Tissue analysis
Modern morphological techniques in molecular cell analysis (Immunocytochemistry autoradiography in situ hybridisation) L. Gordon and A. Bishop
An overview of methodologies for assessing proliferation and cell death R. Dover
10. Chromosomal and molecular assays
11. Assessing proteins and nucleic acids quantitatively and qualitatively by gel fractionation and related techniques
12. Extraction of protein, western blotting, special protein assays - RIA and ELISA
13.Extraction of RNA and northern blot and RT PCR
14.Extraction of mammalian DNA, PCR, Southern blot and dot blot
15. Production of primers/probes J. Jankowski and F. Bedford
16. Molecular cytogenetic analysis of tumours and cell lines and its application in biology and medicine
17. Cytogenetics, FISH, allelle loss S. Jhanwar
Analysis of DNA sequence polymorphisms and screening for point mutations A. Wright and J. Prosser
18. D. Gene manipulation
19. Molecular level
20. Basic cloning techniques
21. Purification of bacterial, plasmid or phage DNA cloning in prokaryotes and construction of recombinant DNA molecules S. Legon
Creation and screening of cDNA libraries
22. A practical approach to cloning J. Goldenring and I. Modlin
23. Identification and isolation of novel DNA and cDna species F. Bedford and J. Jankowski
24. Manual and automated DNA and RNA sequencing M. Jones
25. Assessment of gene expression N. Bates and H. Hurst
26. Cellular level
27. Delivery of DNA into cells
28. Production of competent cells and cell transfection S. Castledon and R. Vile
29. Antisense RNA technology (Oligodeoxyribonucleotides, ribozymes and retroviruses) P. L. Iverson and I. Baysever
30. IV. Trouble-shooting: (i) Microbial contamination during cell culture K. Henderson
31.(ii) Safety aspects of genetic manipulation work S. Legon
32. (iii) tissue storage and preparation J. Jankowski
33. (iv) Failed experiments - general guidance J. Jankowski
34. (v) Selection of adequate controls J. Jankowski
35. (vi) Quality control J. Jankowski
36. V. Gene manipulation and applications to molecular medicine:
37. Inherited genetic disease and developmental disorders P. Buss
38. Oncology of solid tumours J. Jankowski
39. Oncology of haematogenous cancers S. Ridge
40. genetic therapies K. Sikora
VI. Appendices: A. Selected bibliography
B. Commercial suppliers
41. Equipment (quantitative gel analysis and gene manipulation)
42. Consumables
43. Solutions.
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